Coding
CheZ-GFP

Part:BBa_K1709002:Design

Designed by: Astrid Deryckere   Group: iGEM15_KU_Leuven   (2015-09-10)


CheZ-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1289


Design Notes

1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.


Source

DNA sequences originate from the E. Coli genome and previous biobrick sequences.

References